Regulatory

Part:BBa_J100250:Design

Designed by: Jeffrey Burton   Group: Campbell_M_Lab   (2016-01-28)


trp promotor lab


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 26
    Illegal SpeI site found at 34
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 26
    Illegal SpeI site found at 34
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 26
    Illegal SpeI site found at 34
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 26
    Illegal SpeI site found at 34
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We had to take into consideration the natural production of tryptophan by the promoter which acts as a repressor when there are high concentrations of tryptophan.


Source

http://www.ncbi.nlm.nih.gov/pubmed/11252809 where we found the TRP promoter and the -35 to -10 sequence

http://www.hammiverse.com/lectures/18/4.html details of the negative loop mechanism

References